Trainer Philip Hobbs had hoped to blow the cobwebs away in the Masterson Holdings Hurdle before tougher targets later in the season.
The JP McManus-owned gelding was one of the stars of the last campaign, winning all seven starts and following up his Festival win at Aintree.
Speaking to www.gloucestershirelive.co.uk, Hobbs said: "Defi Du Seuil doesn't run.
"It's just watered ground and we don't want to start him on a faster surface than usual.
"It's a long season ahead - it's no good taking risks at this time of year.
"He might go to the Fighting Fifth (December 2) or the International Hurdle (December 16) at Cheltenham."